| Duracell | Chicago, Bethel | 1930 |
| DYMO Corporation | Berkeley | 1958 |
| E-ZPass | Wilmington | 1987 |
| EA Sports | Burnaby | 1991 |
| EarthLink | Atlanta, Pasadena, Atwater Village | 1994 |
| Eastman Chemical Company | Kingsport | 1920 |
| Eastman Kodak | Rochester, Nova Iorque | 1882 |
| Eazel | Mountain View | 1999 |
| EBay | São José | 3 de setembro de 1995 |
| EBSCO Information Services | Birmingham | 1943 |
| Ecolab | Saint Paul | 1923 |
| Edison Manufacturing Company | Nova Iorque | 1889 |
| Edmodo | San Mateo | 1 de setembro de 2008 |
| Elliott Management Corporation | Nova Iorque | 1977 |
| EMachines | Irvine | setembro 1998 |
| Embarcadero Technologies | Austin | outubro 1993 |
| Energy Transfer | Dallas | 1996 |
| Ensoniq | Malvern | 1982 |
| EPAM Systems | Newtown, Pensilvânia | 1993 |
| Equifax | Atlanta | 1899 |
| ESPN+ | Estados Unidos | 12 de abril de 2018 |
| Estúdios Keystone | Edendale | 1912 |
| Eurasia Group | Nova Iorque | 1998 |
| Exelon | Chicago | 2000 |
| Exidy | Sunnyvale | 1974 |
| Expedia | Seattle | 22 de outubro de 1996 |
| Expeditors International | Seattle | 1979 |
| Extreme Championship Wrestling | Filadélfia | 25 de abril de 1992 |
| Exxon | Las Colinas | 1 de janeiro de 1973 |
| ExxonMobil | Spring | 5 de agosto de 1882 |
| FactSet | Norwalk | 1978 |
| Fannie Mae | Noroeste de Washington, D.C. | 1938 |
| Fantasy Flight Games | Roseville | 1995 |
| FedEx | Memphis | 1971 |
| FEI Company | Hillsboro | 1971 |
| Fenty Beauty | 425 Market Street | 8 de setembro de 2018 |
| Fenway Sports Group | Boston | 2001 |
| Fidelity Investments | Boston | 1946 |
| Firefly Aerospace | Cedar Park | 2014 |
| Firehouse Subs | — | 1994 |
| First Citizens BancShares | Raleigh | 7 de agosto de 1986 |
| Fiserv | Brookfield | 1984 |
| Fisher-Price | Nova Iorque | 19 de junho de 1930 |
| Flex LTD | São José, Singapura | 1969 |
| Fluke Corporation | Everett | 1948 |
| Fluor Corporation | Irving | 1890 |
| FMC Technologies | Houston | 2000 |
| Fodera | Nova Iorque | 1983 |